Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3719598..3720195 Replicon chromosome
Accession NZ_CP026157
Organism Klebsiella pneumoniae strain F93-2

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag C2M12_RS18515 Protein ID WP_004142563.1
Coordinates 3719878..3720195 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag C2M12_RS18510 Protein ID WP_004142561.1
Coordinates 3719598..3719885 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2M12_RS18480 3715678..3715926 + 249 WP_002893037.1 DUF1158 domain-containing protein -
C2M12_RS18485 3715944..3716285 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
C2M12_RS18490 3716316..3717431 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
C2M12_RS18495 3717611..3718192 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
C2M12_RS18500 3718192..3718560 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
C2M12_RS18505 3718680..3719333 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
C2M12_RS18510 3719598..3719885 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
C2M12_RS18515 3719878..3720195 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C2M12_RS18520 3720380..3721423 - 1044 WP_023284821.1 DUF2157 domain-containing protein -
C2M12_RS18530 3722092..3722958 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
C2M12_RS18535 3723067..3724494 + 1428 WP_023284820.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T93540 WP_004142563.1 NZ_CP026157:c3720195-3719878 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T93540 NZ_CP026157:c3720195-3719878 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT93540 WP_004142561.1 NZ_CP026157:c3719885-3719598 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT93540 NZ_CP026157:c3719885-3719598 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References