Detailed information of TA system TA093479

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14183..14436
• Replicon: plasmid pF132_2
• Accession: NZ_CP026147
• Organism: Klebsiella pneumoniae strain F132

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: C2M08_RS28525
• Protein ID: WP_001312851.1
• Coordinates: 14183..14332 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	14377..14436 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2M08_RS28480	9542..9957	-	416	Protein_12	IS1 family transposase	-
C2M08_RS28485	10206..10607	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
C2M08_RS28490	10540..10797	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
C2M08_RS28495	10890..11543	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
C2M08_RS28505	12482..13339	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
C2M08_RS28510	13332..13406	-	75	WP_001375168.1	RepA leader peptide Tap	-
C2M08_RS28520	13651..13899	-	249	WP_000083837.1	replication regulatory protein RepA	-
C2M08_RS28525	14183..14332	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	14377..14436	+	60	NuclAT_0	-	Antitoxin
-	14377..14436	+	60	NuclAT_0	-	Antitoxin
-	14377..14436	+	60	NuclAT_0	-	Antitoxin
-	14377..14436	+	60	NuclAT_0	-	Antitoxin
C2M08_RS28535	14637..14867	-	231	WP_001736714.1	hypothetical protein	-
C2M08_RS28540	15031..15630	-	600	WP_032083981.1	hypothetical protein	-
C2M08_RS28545	16016..16216	-	201	WP_015059022.1	hypothetical protein	-
C2M08_RS28550	16348..16908	-	561	WP_000139328.1	fertility inhibition protein FinO	-
C2M08_RS28555	16963..17709	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
C2M08_RS28560	17729..17890	-	162	Protein_25	DNA helicase	-
C2M08_RS28565	17954..18658	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C2M08_RS28570	18711..18776	+	66	Protein_27	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / catA2 / blaKPC-2 / blaSHV-12	-	1..71854	71854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T93479 WP_001312851.1 NZ_CP026147:c14332-14183 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T93479 NZ_CP026147:c14332-14183 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT93479 NZ_CP026147:14377-14436 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin