Detailed information of TA system TA093407

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 102843..103096
• Replicon: plasmid pF5_1
• Accession: NZ_CP026133
• Organism: Klebsiella pneumoniae strain F5

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: C2L61_RS28710
• Protein ID: WP_001312851.1
• Coordinates: 102947..103096 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	102843..102902 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2L61_RS28665	98503..98568	-	66	Protein_128	helix-turn-helix domain-containing protein	-
C2L61_RS28670	98621..99325	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C2L61_RS28675	99389..99550	+	162	Protein_130	DNA helicase	-
C2L61_RS28680	99570..100316	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
C2L61_RS28685	100371..100931	+	561	WP_000139328.1	fertility inhibition protein FinO	-
C2L61_RS28690	101063..101263	+	201	WP_015059022.1	hypothetical protein	-
C2L61_RS28695	101649..102248	+	600	WP_032083981.1	hypothetical protein	-
C2L61_RS28700	102412..102642	+	231	WP_001736714.1	hypothetical protein	-
-	102843..102902	-	60	NuclAT_0	-	Antitoxin
-	102843..102902	-	60	NuclAT_0	-	Antitoxin
-	102843..102902	-	60	NuclAT_0	-	Antitoxin
-	102843..102902	-	60	NuclAT_0	-	Antitoxin
C2L61_RS28710	102947..103096	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
C2L61_RS28715	103380..103628	+	249	WP_000083837.1	replication regulatory protein RepA	-
C2L61_RS28725	103873..103947	+	75	WP_001375168.1	RepA leader peptide Tap	-
C2L61_RS28730	103940..104797	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaKPC-2 / blaSHV-12 / catA2	-	1..105124	105124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T93407 WP_001312851.1 NZ_CP026133:102947-103096 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T93407 NZ_CP026133:102947-103096 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT93407 NZ_CP026133:c102902-102843 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin