Detailed information of TA system TA093360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 21694..22156
• Replicon: plasmid pABA-2f10
• Accession: NZ_CP026129
• Organism: Acinetobacter baumannii strain ABNIH28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3R9PS35
• Locus tag: C2U32_RS19625
• Protein ID: WP_000033474.1
• Coordinates: 21694..21882 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U32_RS19630
• Protein ID: WP_001258992.1
• Coordinates: 21869..22156 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U32_RS19580	16788..17144	+	357	WP_000269901.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C2U32_RS19585	17137..17439	+	303	WP_001140619.1	XRE family transcriptional regulator	-
C2U32_RS19590	17432..17617	+	186	WP_103270968.1	hypothetical protein	-
C2U32_RS19600	19061..19288	-	228	WP_000286812.1	hypothetical protein	-
C2U32_RS19610	20047..20592	-	546	Protein_24	IS5-like element ISAba13 family transposase	-
C2U32_RS19615	20520..20909	-	390	WP_002072923.1	AAA family ATPase	-
C2U32_RS19620	21097..21417	+	321	WP_000088946.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C2U32_RS19625	21694..21882	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U32_RS19630	21869..22156	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	Antitoxin
C2U32_RS19635	22235..22489	-	255	WP_000236586.1	hypothetical protein	-
C2U32_RS19640	22440..22664	-	225	WP_004882762.1	hypothetical protein	-
C2U32_RS19645	22688..23245	-	558	Protein_31	hypothetical protein	-
C2U32_RS19650	23285..24597	-	1313	Protein_32	terminase	-
C2U32_RS19655	24581..25122	-	542	Protein_33	DUF2280 domain-containing protein	-
C2U32_RS19660	25182..25823	-	642	Protein_34	hypothetical protein	-
C2U32_RS19665	25792..26259	-	468	WP_000378524.1	hypothetical protein	-
C2U32_RS19670	26321..26776	-	456	WP_004882767.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..130044	130044
	-	inside	IScluster/Tn	-	-	13021..20472	7451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-90)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7137.56 Da
• Isoelectric Point: 10.9048

>T93360 WP_000033474.1 NZ_CP026129:21694-21882 [Acinetobacter baumannii]
MSKGLFELRLKSQEGIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 189 bp

>T93360 NZ_CP026129:21694-21882 [Acinetobacter baumannii]
ATGAGTAAAGGACTATTTGAGTTGCGCTTAAAATCTCAAGAAGGTATTGCTCGTGTTATGTATTGCACACTAGTCGGTAA
ACGCATTGTAATGCTCCATAGTTTTGTTAAGAAAACCCAAAAAACACCTAAGCAAGATCTAAACCTAGCACTTGACCGCA
TGAAAGAGGTGAAAAATGCGAACACATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10563.32 Da
• Isoelectric Point: 9.7308

>AT93360 WP_001258992.1 NZ_CP026129:21869-22156 [Acinetobacter baumannii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT93360 NZ_CP026129:21869-22156 [Acinetobacter baumannii]
ATGCGAACACATGAAGAAATGAAAGCACTTGCATTATCTCGTTCAGCAGTGCGTACCGAATACGAACGAATTGAACGTGA
AGAAATGCCCTTGCTCGATATGGTACTTACTGCACTTCGAGAGGCAGGTCTATCCCAAGCGCAAATCGCAGAACGCATGG
GAACAAATGTACCTGCCGTTTCACGGCTAGAAAAAGCATTGATCACAGGCAAACCATCACCATCTATTGCCACATTGCAA
AAATATGCTGCGGCAATTGGTAAACATGTTGAAGTGCGTTTTGTCTAA

Structures

Toxin

• AlphaFold DB: A0A3R9PS35