Detailed information of TA system TA093358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 8774..9359
• Replicon: plasmid pABA-6973
• Accession: NZ_CP026126
• Organism: Acinetobacter baumannii strain ABNIH28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: C2U32_RS18655
• Protein ID: WP_000897309.1
• Coordinates: 9039..9359 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U32_RS18650
• Protein ID: WP_000369780.1
• Coordinates: 8774..9046 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U32_RS18620	4018..4446	+	429	WP_016653412.1	hypothetical protein	-
C2U32_RS18625	4512..4703	-	192	WP_000102616.1	hypothetical protein	-
C2U32_RS18635	6113..7042	+	930	WP_005133531.1	replication initiation protein RepM	-
C2U32_RS18640	7121..7645	+	525	WP_005127114.1	plasmid replication DNA-binding protein	-
C2U32_RS18645	7983..8300	+	318	WP_005065053.1	hypothetical protein	-
C2U32_RS18650	8774..9046	-	273	WP_000369780.1	helix-turn-helix domain-containing protein	Antitoxin
C2U32_RS18655	9039..9359	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U32_RS18660	9546..9743	-	198	WP_000476222.1	hypothetical protein	-
C2U32_RS18665	9810..10037	-	228	WP_000921865.1	hypothetical protein	-
C2U32_RS18670	10135..10350	-	216	WP_000071892.1	cold-shock protein	-
C2U32_RS18675	10685..10894	-	210	WP_000069475.1	hypothetical protein	-
C2U32_RS18680	10887..11189	-	303	WP_001129974.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11305	11305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T93358 WP_000897309.1 NZ_CP026126:c9359-9039 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T93358 NZ_CP026126:c9359-9039 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10122.63 Da
• Isoelectric Point: 8.6582

>AT93358 WP_000369780.1 NZ_CP026126:c9046-8774 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT93358 NZ_CP026126:c9046-8774 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAGCGTAAGGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAATTACCGGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCTGAATTTGCTGCAC
GTTTGCATATCAGCGCGAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCGACTGGTCCTGCAGCGACTTTAATT
CGTATTTTAGATGCTCATCCAACTTTAATTTAA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3