Detailed information of TA system TA093324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2928517..2929116
• Replicon: chromosome
• Accession: NZ_CP026105
• Organism: Paraburkholderia hospita strain DSM 17164

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: C2L64_RS13325
• Protein ID: WP_201762687.1
• Coordinates: 2928838..2929116 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A244DXM4
• Locus tag: C2L64_RS13320
• Protein ID: WP_007588944.1
• Coordinates: 2928517..2928825 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2L64_RS13310	2923631..2925547	+	1917	WP_167449594.1	SMP-30/gluconolactonase/LRE family protein	-
C2L64_RS13315	2925986..2927956	+	1971	WP_090839055.1	alkaline phosphatase family protein	-
C2L64_RS53205	2928040..2928204	-	165	WP_158660509.1	hypothetical protein	-
C2L64_RS13320	2928517..2928825	-	309	WP_007588944.1	HigA family addiction module antidote protein	Antitoxin
C2L64_RS13325	2928838..2929116	-	279	WP_201762687.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2L64_RS13330	2929167..2929739	+	573	WP_081498892.1	YdaU family protein	-
C2L64_RS13335	2929703..2930302	-	600	WP_090839057.1	NADPH-dependent F420 reductase	-
C2L64_RS13340	2930405..2931139	-	735	WP_090839062.1	SDR family oxidoreductase	-
C2L64_RS13345	2931270..2931845	+	576	WP_035538937.1	TetR/AcrR family transcriptional regulator	-
C2L64_RS13355	2932590..2933746	-	1157	Protein_2591	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10529.05 Da
• Isoelectric Point: 9.2613

>T93324 WP_201762687.1 NZ_CP026105:c2929116-2928838 [Paraburkholderia hospita]
MIQTFRCPDTQALFEGRRVPRFANIRTVAERKLTLLHAAMTLDALRSPPGNRLEALKGDRAGQYSIRINDQWRICFGWTA
DGPTNVEIVDYH

Nucleotide

• Length: 279 bp

>T93324 NZ_CP026105:c2929116-2928838 [Paraburkholderia hospita]
ATGATCCAAACGTTCCGATGCCCCGATACACAGGCCCTGTTTGAAGGGCGCCGCGTTCCGAGATTTGCGAACATCCGGAC
TGTTGCCGAACGTAAGCTGACACTGCTCCACGCGGCCATGACGCTCGACGCGTTGAGGTCGCCGCCGGGCAACCGGCTCG
AAGCGCTTAAGGGCGACCGCGCCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTTGGATGGACGGCC
GACGGCCCGACGAACGTTGAAATCGTCGACTACCACTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11611.47 Da
• Isoelectric Point: 8.5682

>AT93324 WP_007588944.1 NZ_CP026105:c2928825-2928517 [Paraburkholderia hospita]
MVKNGMRPVHPGEILREEYLVPLGMSANALATALHVTAGRINDIVRERRGITPDTALRLARYFGGDAQTWMNLQLMYDMK
IAERDIARKIEREIEPRELAHA

Nucleotide

• Length: 309 bp

>AT93324 NZ_CP026105:c2928825-2928517 [Paraburkholderia hospita]
ATGGTCAAGAACGGTATGCGCCCGGTCCACCCAGGCGAGATCCTCCGCGAGGAATACCTCGTGCCGCTCGGCATGAGCGC
GAATGCGCTGGCGACGGCGTTGCACGTTACCGCCGGACGGATCAACGACATCGTGCGCGAGCGCCGCGGCATCACGCCGG
ACACTGCATTGCGCCTGGCGCGATATTTCGGCGGCGACGCGCAGACGTGGATGAACCTGCAACTCATGTACGACATGAAG
ATTGCCGAGCGCGACATTGCACGGAAAATCGAGCGCGAAATCGAGCCGCGCGAACTGGCGCACGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A244DXM4