Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3776044..3776662 Replicon chromosome
Accession NZ_CP026098
Organism Shigella flexneri strain FDAARGOS_74

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MC63_RS19545 Protein ID WP_001291435.1
Coordinates 3776444..3776662 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag MC63_RS19540 Protein ID WP_000344797.1
Coordinates 3776044..3776418 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC63_RS19530 (3771133) 3771133..3772326 + 1194 WP_024260158.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MC63_RS19535 (3772349) 3772349..3775498 + 3150 WP_001132472.1 efflux RND transporter permease AcrB -
MC63_RS19540 (3776044) 3776044..3776418 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
MC63_RS19545 (3776444) 3776444..3776662 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MC63_RS19550 (3776834) 3776834..3777385 + 552 WP_000102569.1 maltose O-acetyltransferase -
MC63_RS19555 (3777501) 3777501..3777971 + 471 WP_000136192.1 YlaC family protein -
MC63_RS19560 (3778135) 3778135..3779685 + 1551 WP_025758198.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MC63_RS19565 (3779727) 3779727..3780080 - 354 WP_000878140.1 DUF1428 domain-containing protein -
MC63_RS19575 (3780459) 3780459..3780770 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3780800..3782128 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T93306 WP_001291435.1 NZ_CP026098:3776444-3776662 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T93306 NZ_CP026098:3776444-3776662 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT93306 WP_000344797.1 NZ_CP026098:3776044-3776418 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT93306 NZ_CP026098:3776044-3776418 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References