Detailed information of TA system TA093302

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2664035..2664260
• Replicon: chromosome
• Accession: NZ_CP026098
• Organism: Shigella flexneri strain FDAARGOS_74

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: MC63_RS13605
• Protein ID: WP_000813254.1
• Coordinates: 2664035..2664190 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2664202..2664260 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC63_RS13570	2659294..2659659	+	366	WP_000124119.1	hypothetical protein	-
MC63_RS13575	2659659..2660846	+	1188	Protein_2591	IS91 family transposase	-
MC63_RS13580	2661258..2661812	-	555	WP_000640143.1	DUF1133 family protein	-
MC63_RS13585	2661809..2662099	-	291	WP_000228038.1	DUF1364 domain-containing protein	-
MC63_RS13590	2662099..2662698	-	600	WP_000940329.1	DUF1367 family protein	-
MC63_RS13595	2662832..2663529	+	698	WP_225620307.1	IS1 family transposase	-
MC63_RS13605	2664035..2664190	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2664202..2664260	+	59	-	-	Antitoxin
MC63_RS13610	2664645..2665010	-	366	WP_000610655.1	DUF551 domain-containing protein	-
MC63_RS25720	2665010..2665675	-	666	WP_005115317.1	hypothetical protein	-
MC63_RS13620	2665675..2666037	-	363	Protein_2599	HNH endonuclease	-
MC63_RS13625	2666039..2666257	-	219	WP_000256997.1	DUF4014 family protein	-
MC63_RS13630	2666350..2666706	-	357	WP_000403784.1	hypothetical protein	-
MC63_RS13635	2666764..2667186	-	423	WP_001118171.1	DUF977 family protein	-
MC63_RS13640	2667201..2667947	-	747	WP_000788996.1	ATP-binding protein	-
MC63_RS25725	2668093..2668262	-	170	Protein_2604	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2655005..2682834	27829

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T93302 WP_000813254.1 NZ_CP026098:c2664190-2664035 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T93302 NZ_CP026098:c2664190-2664035 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT93302 NZ_CP026098:2664202-2664260 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin