Detailed information of TA system TA093292

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2150391..2150616
• Replicon: chromosome
• Accession: NZ_CP026098
• Organism: Shigella flexneri strain FDAARGOS_74

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: MC63_RS10660
• Protein ID: WP_252988331.1
• Coordinates: 2150461..2150616 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2150391..2150449 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC63_RS10615	2145702..2146320	-	619	Protein_2031	tyrosine-type recombinase/integrase	-
MC63_RS10620	2146383..2147539	+	1157	WP_094099498.1	IS3-like element IS600 family transposase	-
MC63_RS10625	2147579..2148019	-	441	Protein_2033	phage integrase N-terminal SAM-like domain-containing protein	-
MC63_RS10630	2148019..2148222	-	204	WP_025759339.1	DUF4224 domain-containing protein	-
MC63_RS10635	2148281..2148595	-	315	WP_001317924.1	3'-5' exonuclease	-
MC63_RS10645	2149393..2149809	+	417	WP_005069274.1	hypothetical protein	-
-	2150391..2150449	-	59	-	-	Antitoxin
MC63_RS10660	2150461..2150616	+	156	WP_252988331.1	type I toxin-antitoxin system toxin HokD	Toxin
MC63_RS10670	2150784..2151062	+	279	WP_000929754.1	hypothetical protein	-
MC63_RS10675	2151064..2152122	+	1059	WP_025759819.1	DUF968 domain-containing protein	-
MC63_RS10680	2152123..2152488	+	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
MC63_RS10685	2152485..2153200	+	716	Protein_2042	bacteriophage antitermination protein Q	-
MC63_RS10715	2153996..2154211	+	216	WP_000839572.1	class II holin family protein	-
MC63_RS10720	2154310..2154984	+	675	WP_001088287.1	IS66-like element accessory protein TnpA	-
MC63_RS10725	2154981..2155328	+	348	WP_000631711.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	2099825..2195515	95690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5706.86 Da
• Isoelectric Point: 6.1531

>T93292 WP_252988331.1 NZ_CP026098:2150461-2150616 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRAGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T93292 NZ_CP026098:2150461-2150616 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAGCCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT93292 NZ_CP026098:c2150449-2150391 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin