Detailed information of TA system TA093291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1378849..1379576
• Replicon: chromosome
• Accession: NZ_CP026098
• Organism: Shigella flexneri strain FDAARGOS_74

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2Y4XNA6
• Locus tag: MC63_RS06835
• Protein ID: WP_000547562.1
• Coordinates: 1378849..1379160 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MC63_RS06840
• Protein ID: WP_000126288.1
• Coordinates: 1379157..1379576 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC63_RS06805 (1373998)	1373998..1375683	+	1686	WP_025758592.1	formate hydrogenlyase subunit HycE	-
MC63_RS06810 (1375693)	1375693..1376235	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
MC63_RS06815 (1376235)	1376235..1377002	+	768	WP_025758590.1	formate hydrogenlyase subunit HycG	-
MC63_RS06820 (1376999)	1376999..1377409	+	411	WP_001291916.1	formate hydrogenlyase assembly protein HycH	-
MC63_RS06825 (1377402)	1377402..1377872	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
MC63_RS06830 (1377933)	1377933..1378676	+	744	WP_024259998.1	hypothetical protein	-
MC63_RS06835 (1378849)	1378849..1379160	+	312	WP_000547562.1	type II toxin-antitoxin system HigB family toxin	Toxin
MC63_RS06840 (1379157)	1379157..1379576	+	420	WP_000126288.1	helix-turn-helix domain-containing protein	Antitoxin
MC63_RS06845 (1379691)	1379691..1381115	-	1425	WP_025758587.1	6-phospho-beta-glucosidase AscB	-
MC63_RS06850 (1381124)	1381124..1382581	-	1458	WP_001107865.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
MC63_RS06855 (1382841)	1382841..1383851	+	1011	WP_005051459.1	DNA-binding transcriptional regulator AscG	-
MC63_RS06860 (1384000)	1384000..1384527	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12298.98 Da
• Isoelectric Point: 9.4924

>T93291 WP_000547562.1 NZ_CP026098:1378849-1379160 [Shigella flexneri]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T93291 NZ_CP026098:1378849-1379160 [Shigella flexneri]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAGGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15397.33 Da
• Isoelectric Point: 4.3648

>AT93291 WP_000126288.1 NZ_CP026098:1379157-1379576 [Shigella flexneri]
MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT93291 NZ_CP026098:1379157-1379576 [Shigella flexneri]
ATGACTGCTAATGCTGCACACGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A2Y4XNA6