Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1006820..1007547 Replicon chromosome
Accession NZ_CP026098
Organism Shigella flexneri strain FDAARGOS_74

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag MC63_RS04990 Protein ID WP_000550189.1
Coordinates 1006820..1007134 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MC63_RS04995 Protein ID WP_000560266.1
Coordinates 1007131..1007547 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC63_RS04970 (1002965) 1002965..1003963 - 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -
MC63_RS04975 (1004042) 1004042..1004734 - 693 WP_000942537.1 vancomycin high temperature exclusion protein -
MC63_RS04980 (1004811) 1004811..1005314 - 504 WP_005071012.1 M48 family metallopeptidase -
MC63_RS04985 (1005399) 1005399..1006535 + 1137 WP_000018657.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
MC63_RS04990 (1006820) 1006820..1007134 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
MC63_RS04995 (1007131) 1007131..1007547 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MC63_RS05000 (1007592) 1007592..1009610 - 2019 WP_040235166.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
MC63_RS05005 (1009836) 1009836..1012187 - 2352 WP_000695506.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T93289 WP_000550189.1 NZ_CP026098:1006820-1007134 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T93289 NZ_CP026098:1006820-1007134 [Shigella flexneri]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT93289 WP_000560266.1 NZ_CP026098:1007131-1007547 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT93289 NZ_CP026098:1007131-1007547 [Shigella flexneri]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATCGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References