Detailed information of TA system TA093281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2720..3371
• Replicon: plasmid p2_005069
• Accession: NZ_CP026088
• Organism: Acinetobacter pittii strain WCHAP005069

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: C2U64_RS01140
• Protein ID: WP_000269904.1
• Coordinates: 2720..3076 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U64_RS01145
• Protein ID: WP_001129974.1
• Coordinates: 3069..3371 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U64_RS01125	1..924	+	924	WP_000845851.1	replication initiation protein RepM	-
C2U64_RS01130	992..1537	+	546	WP_000736775.1	plasmid replication DNA-binding protein	-
C2U64_RS01135	1875..2192	+	318	WP_000900860.1	hypothetical protein	-
C2U64_RS01140	2720..3076	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U64_RS01145	3069..3371	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
C2U64_RS01150	3364..3573	+	210	WP_000069475.1	hypothetical protein	-
C2U64_RS01155	3748..4266	+	519	WP_000447195.1	sel1 repeat family protein	-
C2U64_RS01160	4305..4817	+	513	WP_001089574.1	SMI1/KNR4 family protein	-
C2U64_RS01165	5206..6693	+	1488	WP_002115244.1	SulP family inorganic anion transporter	-
C2U64_RS01170	6706..7557	+	852	WP_002115246.1	universal stress protein	-
C2U64_RS01175	7644..7835	-	192	WP_000102616.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9203	9203

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T93281 WP_000269904.1 NZ_CP026088:2720-3076 [Acinetobacter pittii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T93281 NZ_CP026088:2720-3076 [Acinetobacter pittii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT93281 WP_001129974.1 NZ_CP026088:3069-3371 [Acinetobacter pittii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT93281 NZ_CP026088:3069-3371 [Acinetobacter pittii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1