Detailed information of TA system TA093280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 68459..69062
• Replicon: plasmid p1_005069
• Accession: NZ_CP026087
• Organism: Acinetobacter pittii strain WCHAP005069

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C2U64_RS00995
• Protein ID: WP_000288004.1
• Coordinates: 68459..68788 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U64_RS01000
• Protein ID: WP_001258993.1
• Coordinates: 68775..69062 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U64_RS00955	64261..64589	+	329	Protein_53	DUF4236 domain-containing protein	-
C2U64_RS00960	64831..65343	-	513	WP_000464612.1	Fic family protein	-
C2U64_RS00965	65346..65531	-	186	WP_000755724.1	hypothetical protein	-
C2U64_RS00970	65698..66630	+	933	WP_000743263.1	IS5 family transposase	-
C2U64_RS00975	66761..66958	-	198	WP_025464206.1	hypothetical protein	-
C2U64_RS00980	67051..67269	-	219	WP_000994925.1	hypothetical protein	-
C2U64_RS00985	67326..67928	-	603	WP_000096286.1	hypothetical protein	-
C2U64_RS00990	68135..68404	+	270	WP_000453245.1	transcriptional regulator	-
C2U64_RS00995	68459..68788	+	330	WP_000288004.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U64_RS01000	68775..69062	+	288	WP_001258993.1	helix-turn-helix domain-containing protein	Antitoxin
C2U64_RS01005	69099..69287	-	189	WP_000340067.1	hypothetical protein	-
C2U64_RS01010	69696..70019	-	324	Protein_64	DUF4236 domain-containing protein	-
C2U64_RS01015	70028..70204	-	177	WP_002120129.1	hypothetical protein	-
C2U64_RS20885	70276..70599	-	324	WP_005804845.1	hypothetical protein	-
C2U64_RS01025	70954..71463	-	510	Protein_67	Terminase-like domain protein	-
C2U64_RS01030	71447..71970	-	524	Protein_68	DUF2280 domain-containing protein	-
C2U64_RS01035	72061..72702	-	642	WP_000372120.1	hypothetical protein	-
C2U64_RS01040	72671..73132	-	462	WP_000378528.1	hypothetical protein	-
C2U64_RS01045	73193..73648	-	456	WP_001136766.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91563	91563
	-	flank	IS/Tn	-	-	65698..66630	932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12571.69 Da
• Isoelectric Point: 8.8102

>T93280 WP_000288004.1 NZ_CP026087:68459-68788 [Acinetobacter pittii]
MYTIEYYSEDVQDEILTLPDGLLARYFNLTDRMVIYGANLGEPHTLPMSKGLFELRLKSQEGIARVMYCTLVGKRIVMLH
SFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 330 bp

>T93280 NZ_CP026087:68459-68788 [Acinetobacter pittii]
ATGTATACAATTGAATATTACAGCGAAGACGTTCAGGATGAGATTCTTACATTACCTGATGGTCTTTTGGCTCGATATTT
TAACTTAACTGACCGTATGGTGATTTACGGCGCAAACTTAGGAGAACCTCACACATTACCAATGAGTAAAGGACTATTTG
AGTTGCGCTTAAAATCTCAAGAAGGTATTGCTCGTGTTATGTATTGCACACTAGTCGGTAAACGCATTGTAATGCTCCAT
AGTTTTGTTAAGAAAACCCAAAAAACACCTAAGCAAGATCTAAACCTAGCACTTGACCGCATGAAAGAGGTGAAAAATGC
GAACACATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10565.30 Da
• Isoelectric Point: 10.1453

>AT93280 WP_001258993.1 NZ_CP026087:68775-69062 [Acinetobacter pittii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTARRDAGLSQAQIAERMGTSVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT93280 NZ_CP026087:68775-69062 [Acinetobacter pittii]
ATGCGAACACATGAAGAAATGAAAGCACTTGCATTATCTCGTTCAGCAGTGCGTACCGAATACGAACGAATTGAACGTGA
AGAAATGCCCCTGCTCGATATGGTACTTACTGCACGTCGAGATGCAGGTCTATCCCAAGCGCAAATCGCGGAACGCATGG
GAACAAGTGTACCTGCCGTTTCACGGCTAGAAAAAGCATTGATCACAGGCAAACCATCACCATCTATTGCCACATTGCAA
AAATATGCTGCGGCAATTGGTAAACATGTTGAAGTGCGTTTTGTCTAA