Detailed information of TA system TA093279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 37008..37617
• Replicon: plasmid p1_005069
• Accession: NZ_CP026087
• Organism: Acinetobacter pittii strain WCHAP005069

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C2U64_RS00880
• Protein ID: WP_000269908.1
• Coordinates: 37261..37617 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U64_RS00875
• Protein ID: WP_002120200.1
• Coordinates: 37008..37268 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U64_RS00840	32481..33479	+	999	WP_002120211.1	zinc-binding alcohol dehydrogenase family protein	-
C2U64_RS00845	33500..33784	+	285	WP_000155817.1	antibiotic biosynthesis monooxygenase	-
C2U64_RS00850	33919..34140	+	222	Protein_34	IS3 family transposase	-
C2U64_RS00860	34433..35758	-	1326	WP_008942541.1	ISNCY family transposase	-
C2U64_RS00865	35972..36199	-	228	WP_000555567.1	hypothetical protein	-
C2U64_RS00875	37008..37268	-	261	WP_002120200.1	helix-turn-helix transcriptional regulator	Antitoxin
C2U64_RS00880	37261..37617	-	357	WP_000269908.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U64_RS00885	38175..38858	+	684	WP_000478902.1	hypothetical protein	-
C2U64_RS00890	39063..39591	+	529	Protein_40	IS3 family transposase	-
C2U64_RS00895	39834..40154	+	321	WP_001081101.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C2U64_RS00900	40425..42380	-	1956	WP_000593543.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91563	91563
	-	inside	IScluster/Tn	-	-	34433..43635	9202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-83)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13622.56 Da
• Isoelectric Point: 6.4651

>T93279 WP_000269908.1 NZ_CP026087:c37617-37261 [Acinetobacter pittii]
MWTVITTDLFNEWLEQQDESTQEKVLTALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPFRQAI
VLCIGDKGGKKRFYKEMLNIAEQQYELHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T93279 NZ_CP026087:c37617-37261 [Acinetobacter pittii]
ATGTGGACAGTCATCACGACAGATCTGTTTAATGAATGGCTTGAACAGCAAGACGAATCAACACAAGAGAAAGTCTTAAC
CGCTTTAGTTGTTTTACAGCAGCAGGGACCAAGTTTAGGTCGCCCTTTAGTTGATACCGTATATGACTCTAAATTTACCA
ATATGAAAGAGCTACGCGTTCAGCATCGAGGAAAACCCCTGAGAGCCTTTTTCGCATTTGATCCATTTCGACAGGCAATT
GTGCTGTGCATCGGTGATAAAGGCGGTAAAAAGCGTTTCTATAAAGAGATGCTGAACATTGCAGAGCAGCAATACGAACT
TCATCTCTCAACCCTTGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9692.11 Da
• Isoelectric Point: 4.8223

>AT93279 WP_002120200.1 NZ_CP026087:c37268-37008 [Acinetobacter pittii]
MAKTLQELLASRSPESQTRIQQMADELLLETQLHFIREELEISQKELAATLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGETPY

Nucleotide

• Length: 261 bp

>AT93279 NZ_CP026087:c37268-37008 [Acinetobacter pittii]
ATGGCTAAAACCTTACAGGAATTATTGGCTAGTCGCTCTCCAGAGAGCCAAACTCGTATTCAACAAATGGCTGACGAGCT
ACTTTTAGAAACTCAGCTTCACTTTATTCGAGAAGAATTAGAGATTTCCCAAAAGGAACTGGCTGCAACTCTTGGAATCA
AACAACCTTCATTGTCAGCAATCGAAAACCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAAGCTATG
GGGGGGGAAACTCCGTATTGA