Detailed information of TA system TA093275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 82855..83440
• Replicon: plasmid pOXA58_005069
• Accession: NZ_CP026086
• Organism: Acinetobacter pittii strain WCHAP005069

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: C2U64_RS00470
• Protein ID: WP_000897307.1
• Coordinates: 83120..83440 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U64_RS00465
• Protein ID: WP_000369781.1
• Coordinates: 82855..83127 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U64_RS00445	78369..79694	-	1326	WP_008942541.1	ISNCY family transposase	-
C2U64_RS00455	79947..80831	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
C2U64_RS00460	80887..82362	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C2U64_RS00465	82855..83127	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
C2U64_RS00470	83120..83440	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U64_RS00480	83564..84889	-	1326	WP_008942541.1	ISNCY family transposase	-
C2U64_RS00490	85068..85487	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
C2U64_RS00495	85567..85848	-	282	WP_000985609.1	putative addiction module antidote protein	-
C2U64_RS00500	85849..86151	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C2U64_RS00505	86300..86830	+	531	WP_000172759.1	hypothetical protein	-
C2U64_RS00510	86919..87233	-	315	WP_004728120.1	BrnA antitoxin family protein	-
C2U64_RS00515	87220..87507	-	288	WP_000438825.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-VIa / mph(E) / msr(E) / blaOXA-58	-	1..112436	112436
	-	inside	IScluster/Tn	mph(E) / msr(E) / blaOXA-58	-	76888..94913	18025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T93275 WP_000897307.1 NZ_CP026086:c83440-83120 [Acinetobacter pittii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T93275 NZ_CP026086:c83440-83120 [Acinetobacter pittii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT93275 WP_000369781.1 NZ_CP026086:c83127-82855 [Acinetobacter pittii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT93275 NZ_CP026086:c83127-82855 [Acinetobacter pittii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3