Detailed information of TA system TA093274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2720..3371
• Replicon: plasmid pOXA58_005069
• Accession: NZ_CP026086
• Organism: Acinetobacter pittii strain WCHAP005069

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: C2U64_RS00020
• Protein ID: WP_000269904.1
• Coordinates: 2720..3076 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U64_RS00025
• Protein ID: WP_001129974.1
• Coordinates: 3069..3371 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U64_RS00005	1..924	+	924	WP_000845851.1	replication initiation protein RepM	-
C2U64_RS00010	992..1537	+	546	WP_000736775.1	plasmid replication DNA-binding protein	-
C2U64_RS00015	1875..2192	+	318	WP_000900860.1	hypothetical protein	-
C2U64_RS00020	2720..3076	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U64_RS00025	3069..3371	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
C2U64_RS00030	3364..3573	+	210	WP_000069475.1	hypothetical protein	-
C2U64_RS00035	3748..4266	+	519	WP_000447195.1	sel1 repeat family protein	-
C2U64_RS00040	4305..4817	+	513	WP_001089574.1	SMI1/KNR4 family protein	-
C2U64_RS00045	5143..5847	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
C2U64_RS00050	6045..6548	+	504	Protein_9	Tn3 family transposase	-
C2U64_RS00055	6654..7514	+	861	WP_000557454.1	aminoglycoside N-acetyltransferase AAC(3)-IId	-
C2U64_RS00060	7527..8069	+	543	WP_000587837.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-VIa / mph(E) / msr(E) / blaOXA-58	-	1..112436	112436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T93274 WP_000269904.1 NZ_CP026086:2720-3076 [Acinetobacter pittii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T93274 NZ_CP026086:2720-3076 [Acinetobacter pittii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT93274 WP_001129974.1 NZ_CP026086:3069-3371 [Acinetobacter pittii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT93274 NZ_CP026086:3069-3371 [Acinetobacter pittii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1