Detailed information of TA system TA093273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4634785..4635387
• Replicon: chromosome
• Accession: NZ_CP026085
• Organism: Escherichia coli strain DH5alpha

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C1467_RS23930
• Protein ID: WP_000897305.1
• Coordinates: 4635076..4635387 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C1467_RS23925
• Protein ID: WP_000356397.1
• Coordinates: 4634785..4635075 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1467_RS23890	4630272..4630907	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C1467_RS23895	4630904..4631833	+	930	WP_000027702.1	formate dehydrogenase accessory protein FdhE	-
C1467_RS23900	4632196..4633404	-	1209	WP_006250222.1	IS4-like element ISVsa5 family transposase	-
C1467_RS23910	4633474..4633743	-	270	WP_158649079.1	hypothetical protein	-
C1467_RS23915	4633962..4634180	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C1467_RS23925	4634785..4635075	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C1467_RS23930	4635076..4635387	-	312	WP_000897305.1	hypothetical protein	Toxin
C1467_RS23935	4635616..4636524	+	909	WP_001162704.1	alpha/beta hydrolase	-
C1467_RS23940	4636588..4637529	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C1467_RS23945	4637574..4638011	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C1467_RS23950	4638008..4638880	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C1467_RS23955	4638874..4639473	-	600	WP_001315111.1	glucose-1-phosphatase	-
C1467_RS23960	4639572..4640357	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4632196..4633404	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T93273 WP_000897305.1 NZ_CP026085:c4635387-4635076 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T93273 NZ_CP026085:c4635387-4635076 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT93273 WP_000356397.1 NZ_CP026085:c4635075-4634785 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT93273 NZ_CP026085:c4635075-4634785 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7