Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3794116..3794657 Replicon chromosome
Accession NZ_CP026085
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag C1467_RS19765 Protein ID WP_000615976.1
Coordinates 3794379..3794657 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C1467_RS19760 Protein ID WP_000729704.1
Coordinates 3794116..3794376 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1467_RS19735 3789152..3790291 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
C1467_RS19740 3790275..3792368 + 2094 WP_001140808.1 flagellar biosynthesis protein FlhA -
C1467_RS19745 3792473..3792751 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C1467_RS19750 3792744..3793100 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
C1467_RS19755 3793157..3793930 - 774 WP_000543895.1 C40 family peptidase -
C1467_RS19760 3794116..3794376 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C1467_RS19765 3794379..3794657 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C1467_RS19770 3794813..3795553 + 741 WP_001225679.1 murein L,D-transpeptidase -
C1467_RS19775 3795524..3796291 - 768 WP_000333380.1 class II glutamine amidotransferase -
C1467_RS19780 3796497..3797075 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3792744..3803685 10941


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T93268 WP_000615976.1 NZ_CP026085:3794379-3794657 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T93268 NZ_CP026085:3794379-3794657 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT93268 WP_000729704.1 NZ_CP026085:3794116-3794376 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT93268 NZ_CP026085:3794116-3794376 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References