Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1869367..1869869 Replicon chromosome
Accession NZ_CP026085
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C1467_RS09635 Protein ID WP_000767829.1
Coordinates 1869615..1869869 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C1467_RS09630 Protein ID WP_001259255.1
Coordinates 1869367..1869618 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1467_RS09600 1864545..1865612 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C1467_RS09605 1865612..1866682 - 1071 WP_000108957.1 histidinol-phosphate transaminase -
C1467_RS09610 1866679..1867983 - 1305 WP_001349967.1 histidinol dehydrogenase -
C1467_RS09615 1867989..1868888 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C1467_RS09625 1869034..1869084 - 51 WP_001364200.1 his operon leader peptide -
C1467_RS09630 1869367..1869618 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C1467_RS09635 1869615..1869869 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C1467_RS09640 1869952..1870776 + 825 WP_000754749.1 SDR family oxidoreductase -
C1467_RS09645 1870822..1871751 + 930 WP_000803351.1 LysR family transcriptional regulator -
C1467_RS09650 1871966..1872028 + 63 WP_010723108.1 membrane protein YoeI -
C1467_RS09655 1872018..1873376 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C1467_RS09660 1873555..1874613 + 1059 WP_000492346.1 transport protein YeeE -
C1467_RS09665 1874627..1874854 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T93254 WP_000767829.1 NZ_CP026085:1869615-1869869 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T93254 NZ_CP026085:1869615-1869869 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT93254 WP_001259255.1 NZ_CP026085:1869367-1869618 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT93254 NZ_CP026085:1869367-1869618 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References