Detailed information of TA system TA093216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1221702..1222219
• Replicon: chromosome
• Accession: NZ_CP026079
• Organism: Staphylococcus aureus strain FDAARGOS_9

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: RK67_RS06120
• Protein ID: WP_000113265.1
• Coordinates: 1221953..1222219 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: RK67_RS06115
• Protein ID: WP_000584499.1
• Coordinates: 1221702..1221953 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK67_RS06085	1217400..1218119	+	720	WP_000479576.1	amino acid ABC transporter permease	-
RK67_RS06090	1218116..1218847	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
RK67_RS06095	1218944..1220194	-	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
RK67_RS06100	1220449..1220811	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
RK67_RS06105	1220830..1221429	+	600	WP_000162810.1	DsbA family protein	-
RK67_RS06110	1221436..1221537	+	102	Protein_1137	hypothetical protein	-
RK67_RS06115	1221702..1221953	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RK67_RS06120	1221953..1222219	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
RK67_RS06125	1222254..1222325	+	72	Protein_1140	hypothetical protein	-
RK67_RS06130	1222514..1224061	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
RK67_RS06135	1224247..1224714	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
RK67_RS06140	1224798..1224980	+	183	WP_000230292.1	hypothetical protein	-
RK67_RS06145	1225197..1226021	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
RK67_RS06150	1226255..1226791	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T93216 WP_000113265.1 NZ_CP026079:1221953-1222219 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T93216 NZ_CP026079:1221953-1222219 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT93216 WP_000584499.1 NZ_CP026079:1221702-1221953 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT93216 NZ_CP026079:1221702-1221953 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60