Detailed information of TA system TA093096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 156220..156643
• Replicon: chromosome
• Accession: NZ_CP026071
• Organism: Staphylococcus aureus strain FDAARGOS_30

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5S9I4Y6
• Locus tag: RK88_RS00940
• Protein ID: WP_001065091.1
• Coordinates: 156220..156471 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0E0VQM8
• Locus tag: RK88_RS00945
• Protein ID: WP_000028422.1
• Coordinates: 156461..156643 (+)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK88_RS00900	151875..152795	+	921	WP_000138472.1	recombinase RecT	-
RK88_RS00905	152876..153493	+	618	WP_071621397.1	MBL fold metallo-hydrolase	-
RK88_RS00910	153494..153964	+	471	WP_000934764.1	single-stranded DNA-binding protein	-
RK88_RS00915	153994..154878	+	885	WP_000148316.1	DnaD domain protein	-
RK88_RS00920	154885..155103	+	219	WP_000338531.1	hypothetical protein	-
RK88_RS00925	155112..155421	+	310	Protein_168	RusA family crossover junction endodeoxyribonuclease	-
RK88_RS00930	155434..155802	+	369	WP_000101274.1	hypothetical protein	-
RK88_RS00935	155806..156048	+	243	WP_000131366.1	hypothetical protein	-
RK88_RS14860	156060..156227	+	168	WP_001624242.1	hypothetical protein	-
RK88_RS00940	156220..156471	+	252	WP_001065091.1	DUF1024 family protein	Toxin
RK88_RS00945	156461..156643	+	183	WP_000028422.1	hypothetical protein	Antitoxin
RK88_RS00950	156636..157178	+	543	WP_000185659.1	dUTP pyrophosphatase	-
RK88_RS00955	157215..157421	+	207	WP_000195803.1	DUF1381 domain-containing protein	-
RK88_RS00960	157418..157804	+	387	WP_000592207.1	hypothetical protein	-
RK88_RS00965	157801..157950	+	150	WP_000595265.1	transcriptional activator RinB	-
RK88_RS00970	157950..158150	+	201	WP_000265043.1	DUF1514 family protein	-
RK88_RS00975	158178..158594	+	417	WP_000590122.1	hypothetical protein	-
RK88_RS00980	158826..159125	+	300	WP_000988336.1	HNH endonuclease	-
RK88_RS00985	159255..159599	+	345	WP_000402904.1	hypothetical protein	-
RK88_RS00990	159596..161257	+	1662	WP_000625088.1	terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL / tsst-1 / hlb / sea / sak / chp / scn	116348..184310	67962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9349.22 Da
• Isoelectric Point: 3.9473

>T93096 WP_001065091.1 NZ_CP026071:156220-156471 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEH

Nucleotide

• Length: 252 bp

>T93096 NZ_CP026071:156220-156471 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTTTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6741.63 Da
• Isoelectric Point: 4.2658

>AT93096 WP_000028422.1 NZ_CP026071:156461-156643 [Staphylococcus aureus]
MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLIDFVVKPIHLIKEEQEND

Nucleotide

• Length: 183 bp

>AT93096 NZ_CP026071:156461-156643 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Structures

Toxin

• AlphaFold DB: A0A5S9I4Y6

Antitoxin

• AlphaFold DB: A0A0E0VQM8