Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 89764..90491 Replicon plasmid unnamed2
Accession NZ_CP026049
Organism Raoultella planticola strain FDAARGOS_64

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag MC50_RS27830 Protein ID WP_004118600.1
Coordinates 89764..90075 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag MC50_RS27835 Protein ID WP_000990392.1
Coordinates 90072..90491 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC50_RS27810 86278..86997 + 720 WP_001102107.1 (Fe-S)-binding protein -
MC50_RS27815 87008..88435 + 1428 WP_000023895.1 iron-sulfur cluster-binding protein -
MC50_RS27820 88428..89123 + 696 WP_004118595.1 lactate utilization protein C -
MC50_RS27825 89173..89553 - 381 Protein_102 gluconate permease -
MC50_RS27830 89764..90075 + 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
MC50_RS27835 90072..90491 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
MC50_RS27840 90528..91730 - 1203 WP_003031541.1 hypothetical protein -
MC50_RS27845 91723..92052 - 330 WP_004118613.1 hypothetical protein -
MC50_RS27850 92049..92708 - 660 WP_000078540.1 hypothetical protein -
MC50_RS28755 93132..93725 - 594 WP_044348674.1 hypothetical protein -
MC50_RS27865 94041..94394 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
MC50_RS27870 94442..94804 + 363 WP_000783215.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..206855 206855


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T92936 WP_004118600.1 NZ_CP026049:89764-90075 [Raoultella planticola]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T92936 NZ_CP026049:89764-90075 [Raoultella planticola]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT92936 WP_000990392.1 NZ_CP026049:90072-90491 [Raoultella planticola]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT92936 NZ_CP026049:90072-90491 [Raoultella planticola]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGATCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References