Detailed information of TA system TA092918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3737871..3738433
• Replicon: chromosome
• Accession: NZ_CP026045
• Organism: Citrobacter freundii strain FDAARGOS_61

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1C0NXV4
• Locus tag: MC47_RS18190
• Protein ID: WP_003833016.1
• Coordinates: 3738155..3738433 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MC47_RS18185
• Protein ID: WP_047411217.1
• Coordinates: 3737871..3738155 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC47_RS18170	3735561..3736445	+	885	WP_003833024.1	formate dehydrogenase N subunit beta	-
MC47_RS18175	3736438..3737094	+	657	WP_003020054.1	formate dehydrogenase-N subunit gamma	-
MC47_RS18180	3737224..3737823	+	600	WP_047411210.1	inorganic diphosphatase	-
MC47_RS18185	3737871..3738155	-	285	WP_047411217.1	HigA family addiction module antidote protein	Antitoxin
MC47_RS18190	3738155..3738433	-	279	WP_003833016.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MC47_RS18195	3738654..3740369	-	1716	WP_047411222.1	ATP-binding cassette domain-containing protein	-
MC47_RS18200	3740834..3741736	+	903	WP_043016304.1	endonuclease/exonuclease/phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10667.30 Da
• Isoelectric Point: 9.8965

>T92918 WP_003833016.1 NZ_CP026045:c3738433-3738155 [Citrobacter freundii]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTDINMPGYKLHPLVGERDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T92918 NZ_CP026045:c3738433-3738155 [Citrobacter freundii]
ATGATCATGAACTTCAGACACAAAGGATTGCGCGATTTGTTTCTTCATGGGCGAACATCAGGCGTTATGGCGAAGCATGT
CCGACGATTACGACACAGGCTTGCCGTTATTGATGCGGCAAGTAAGGTTACAGATATCAATATGCCCGGCTATAAGCTAC
ATCCTCTGGTGGGTGAGCGTGACGGCATATGGGCAATTTCTGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGACGCGTACATACTGGACTACGAGGACTATCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10357.03 Da
• Isoelectric Point: 10.1834

>AT92918 WP_047411217.1 NZ_CP026045:c3738155-3737871 [Citrobacter freundii]
MKMANHPRPGDIIQEALNELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNSWSLAEAQ
KNVDTSRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT92918 NZ_CP026045:c3738155-3737871 [Citrobacter freundii]
ATGAAAATGGCAAATCATCCCCGCCCTGGGGATATCATTCAGGAAGCACTGAACGAACTCAATGTCAGCCTGCGCGAATT
TGCCAGGGCGATGGAGATTGCACCTTCGACGGCAAGCCGACTATTAACCGGGAAAGCGGCCCTGACGCCTGAGATGGCGA
TCAAACTCTCCGTGGTGATTGGCAGTTCACCTGAAATGTGGTTAAACCTGCAAAATAGCTGGAGCCTGGCAGAGGCCCAA
AAAAACGTGGATACTTCACGCCTGCGCAAACTGGCAGTGCAGTGA

Structures

Toxin

• AlphaFold DB: A0A1C0NXV4