Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 1482248..1482914 Replicon chromosome
Accession NZ_CP026045
Organism Citrobacter freundii strain FDAARGOS_61

Toxin (Protein)


Gene name higB Uniprot ID A0A5B0T6K1
Locus tag MC47_RS07285 Protein ID WP_047408175.1
Coordinates 1482597..1482914 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag MC47_RS07280 Protein ID WP_003837894.1
Coordinates 1482248..1482544 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC47_RS07265 1479424..1479897 - 474 WP_016154802.1 transcription elongation factor GreB -
MC47_RS07270 1480123..1480842 + 720 WP_001157751.1 two-component system response regulator OmpR -
MC47_RS07275 1480839..1482191 + 1353 WP_043018579.1 two-component system sensor histidine kinase EnvZ -
MC47_RS07280 1482248..1482544 - 297 WP_003837894.1 helix-turn-helix domain-containing protein Antitoxin
MC47_RS07285 1482597..1482914 - 318 WP_047408175.1 toxin HigB-2 Toxin
MC47_RS07290 1483037..1484659 - 1623 WP_043018578.1 phosphoenolpyruvate carboxykinase (ATP) -
MC47_RS07295 1485237..1486115 - 879 WP_016157640.1 Hsp33 family molecular chaperone HslO -
MC47_RS07300 1486140..1486541 - 402 WP_019078146.1 ribosome-associated heat shock protein Hsp15 -
MC47_RS07305 1486552..1487220 - 669 WP_003023533.1 GMP/IMP nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12255.17 Da        Isoelectric Point: 10.0909

>T92906 WP_047408175.1 NZ_CP026045:c1482914-1482597 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQETLIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T92906 NZ_CP026045:c1482914-1482597 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAAGGATTTAGTAAACGCCGTCCGTTACTTTTGCCTGACGATGAGTTTCGCGCCTTTCA
AGAGACGCTTATAGAGAACCCTGAGGCTGGAGATACTATCGCTGGTACAGGTGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGCAAACGCAGCGGTATTAGGGTTATCTATTACAACGTGACGCGCAAAGGTCGAATCTATCTCGCTTTGCTC
TATCCTAAAAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCAATATGCTGATTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT92906 WP_003837894.1 NZ_CP026045:c1482544-1482248 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp

>AT92906 NZ_CP026045:c1482544-1482248 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTGCGTATAGAGAAAGGTGAAGAAACACCTAA
GCCTGAACATGTACATACCTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATCGCTGTTGGCGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAGCCGACTGGTGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAATCCATTTATAATTAATCAATTAAAAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5B0T6K1

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References