Detailed information of TA system TA092904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 956906..957505
• Replicon: chromosome
• Accession: NZ_CP026045
• Organism: Citrobacter freundii strain FDAARGOS_61

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MC47_RS04730
• Protein ID: WP_047407483.1
• Coordinates: 957194..957505 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MC47_RS04725
• Protein ID: WP_047407480.1
• Coordinates: 956906..957193 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC47_RS04710	954402..955304	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
MC47_RS04715	955301..955936	+	636	WP_047407477.1	formate dehydrogenase cytochrome b556 subunit	-
MC47_RS04720	955933..956862	+	930	WP_043018887.1	formate dehydrogenase accessory protein FdhE	-
MC47_RS04725	956906..957193	-	288	WP_047407480.1	helix-turn-helix domain-containing protein	Antitoxin
MC47_RS04730	957194..957505	-	312	WP_047407483.1	hypothetical protein	Toxin
MC47_RS04735	957726..958634	+	909	WP_047407486.1	alpha/beta hydrolase	-
MC47_RS04740	958699..958941	+	243	WP_003825282.1	type II toxin-antitoxin system ParD family antitoxin	-
MC47_RS04745	958934..959221	+	288	WP_003825284.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MC47_RS04750	959227..960168	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
MC47_RS04755	960213..960650	-	438	WP_003028671.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MC47_RS04760	960647..961519	-	873	WP_047407489.1	virulence factor BrkB family protein	-
MC47_RS04765	961513..962112	-	600	WP_043018883.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12270.39 Da
• Isoelectric Point: 10.0972

>T92904 WP_047407483.1 NZ_CP026045:c957505-957194 [Citrobacter freundii]
MLFIETEIFTEDVKKLLDDDEYRRLQIFLTIQPDCGDLIHDTGGLRKVRWRARGKGKRGGVRIIYFHQIRKSQIRLLLIY
QKGIKDDLTPQEKVLLRMLNEGW

Nucleotide

• Length: 312 bp

>T92904 NZ_CP026045:c957505-957194 [Citrobacter freundii]
ATGCTATTCATAGAAACGGAAATCTTTACTGAAGACGTCAAAAAATTACTGGATGATGATGAATACCGTAGACTACAGAT
ATTCCTTACGATTCAGCCCGATTGTGGCGATCTGATACATGATACAGGGGGCCTGAGAAAGGTTCGCTGGAGAGCGCGAG
GGAAAGGAAAACGTGGCGGTGTAAGGATTATCTACTTTCATCAAATCAGGAAATCACAAATTCGTTTGTTGCTGATTTAT
CAGAAAGGGATCAAGGATGATCTGACTCCACAGGAAAAAGTACTGCTGCGTATGTTGAATGAGGGGTGGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10819.40 Da
• Isoelectric Point: 10.8907

>AT92904 WP_047407480.1 NZ_CP026045:c957193-956906 [Citrobacter freundii]
MDKALFERLTQSMIQMNEIDEGTRQPSRTFHIDAMKIKEIRQSSGLSQTRFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPKHVIQALR

Nucleotide

• Length: 288 bp

>AT92904 NZ_CP026045:c957193-956906 [Citrobacter freundii]
ATGGATAAGGCACTATTTGAGCGATTAACTCAAAGTATGATTCAGATGAATGAGATCGATGAAGGAACGCGGCAGCCTTC
TCGTACCTTTCATATTGACGCCATGAAAATAAAGGAAATCCGCCAGTCTTCAGGATTATCACAGACTCGTTTCGCAGATT
TGATATCGGTAAGCGTGGATACGTTGCGAAATTGGGAGCAGGGGCGTCGTTCACCGACCGGTCCAGCAAAAGCGTTGTTA
CGTGCCATTGCCAATGATCCAAAACATGTCATTCAGGCGCTAAGATAA