Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2087236..2087738 Replicon chromosome
Accession NZ_CP026028
Organism Escherichia coli strain CIT

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C2H83_RS11085 Protein ID WP_000767829.1
Coordinates 2087236..2087490 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C2H83_RS11090 Protein ID WP_001259255.1
Coordinates 2087487..2087738 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2H83_RS11055 2082251..2082478 - 228 WP_000234896.1 sulfurtransferase TusA -
C2H83_RS11060 2082492..2083550 - 1059 WP_000492339.1 transport protein YeeE -
C2H83_RS11065 2083729..2085087 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C2H83_RS11070 2085077..2085139 - 63 WP_010723108.1 membrane protein YoeI -
C2H83_RS11075 2085354..2086283 - 930 WP_000803351.1 LysR family transcriptional regulator -
C2H83_RS11080 2086329..2087153 - 825 WP_000754737.1 SDR family oxidoreductase -
C2H83_RS11085 2087236..2087490 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C2H83_RS11090 2087487..2087738 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C2H83_RS11095 2088021..2088071 + 51 WP_001364200.1 his operon leader peptide -
C2H83_RS11105 2088217..2089116 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C2H83_RS11110 2089122..2090426 + 1305 WP_000009594.1 histidinol dehydrogenase -
C2H83_RS11115 2090423..2091493 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
C2H83_RS11120 2091493..2092560 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92856 WP_000767829.1 NZ_CP026028:c2087490-2087236 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92856 NZ_CP026028:c2087490-2087236 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92856 WP_001259255.1 NZ_CP026028:c2087738-2087487 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92856 NZ_CP026028:c2087738-2087487 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References