Detailed information of TA system TA092694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6299..7023
• Replicon: plasmid p13450-2
• Accession: NZ_CP026016
• Organism: Klebsiella variicola strain 13450

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: C2D62_RS28615
• Protein ID: WP_023292165.1
• Coordinates: 6299..6610 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2D62_RS28620
• Protein ID: WP_072122726.1
• Coordinates: 6607..7023 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2D62_RS28595	2307..3209	-	903	WP_060598839.1	LysR family transcriptional regulator	-
C2D62_RS28600	3492..4631	+	1140	WP_153242761.1	PLP-dependent aspartate aminotransferase family protein	-
C2D62_RS28605	4652..5581	+	930	WP_158236771.1	DMT family transporter	-
C2D62_RS28610	5696..5997	+	302	Protein_5	DDE-type integrase/transposase/recombinase	-
C2D62_RS28615	6299..6610	+	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
C2D62_RS28620	6607..7023	+	417	WP_072122726.1	helix-turn-helix domain-containing protein	Antitoxin
C2D62_RS28625	7840..8220	+	381	WP_077138875.1	hypothetical protein	-
C2D62_RS28630	8286..8633	+	348	WP_077138876.1	hypothetical protein	-
C2D62_RS28635	8721..8951	+	231	WP_004197732.1	hypothetical protein	-
C2D62_RS28640	8998..9831	+	834	WP_077138877.1	type I restriction-modification system subunit M	-
C2D62_RS28645	10456..10986	+	531	WP_077138878.1	antirestriction protein	-
C2D62_RS28650	11024..11503	-	480	WP_077138879.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..109205	109205

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T92694 WP_023292165.1 NZ_CP026016:6299-6610 [Klebsiella variicola]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T92694 NZ_CP026016:6299-6610 [Klebsiella variicola]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCAAGACACTATCCGAATGATGCAGCGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAGAAATGGTGGGTTATCGATATCGGCGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15468.55 Da
• Isoelectric Point: 4.4711

>AT92694 WP_072122726.1 NZ_CP026016:6607-7023 [Klebsiella variicola]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDTWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD

Nucleotide

• Length: 417 bp

>AT92694 NZ_CP026016:6607-7023 [Klebsiella variicola]
ATGATTTTTAGCGATGCCATTAAAGCTGCGAATGACCTGGCCAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCACTTAAACTCGTTGAATATCTTCTGGAACATGATCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATACCTGGGAAGATAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTA
TCGTTGCTGCGGGTTCTTATGCAGCAACACGGGCTTTCACAGTCCGATTTCGAAAATGAGATAGGTAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTTGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3