Detailed information of TA system TA092671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 147721..148448
• Replicon: plasmid pK2044
• Accession: NZ_CP026012
• Organism: Klebsiella pneumoniae strain K2044

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: C2D63_RS25630
• Protein ID: WP_011251285.1
• Coordinates: 148137..148448 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2D63_RS25625
• Protein ID: WP_011251286.1
• Coordinates: 147721..148140 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2D63_RS25605	142867..145836	+	2970	WP_004213592.1	Tn3 family transposase	-
C2D63_RS25610	145878..146372	+	495	WP_004213594.1	hypothetical protein	-
C2D63_RS25615	146433..146549	+	117	Protein_149	transcriptional regulator	-
C2D63_RS25620	146606..147574	-	969	WP_011251287.1	IS5 family transposase	-
C2D63_RS25625	147721..148140	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
C2D63_RS25630	148137..148448	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
C2D63_RS25635	148653..149111	-	459	Protein_153	DDE-type integrase/transposase/recombinase	-
C2D63_RS25640	149225..149922	+	698	Protein_154	IS1 family transposase	-
C2D63_RS25645	149939..150373	+	435	Protein_155	thioredoxin fold domain-containing protein	-
C2D63_RS25650	150390..150701	+	312	WP_011251282.1	hypothetical protein	-
C2D63_RS25655	150715..151056	+	342	WP_011251281.1	hypothetical protein	-
C2D63_RS25660	151116..152072	+	957	WP_011251280.1	DsbA family protein	-
C2D63_RS25665	152189..152764	+	576	WP_011251279.1	hypothetical protein	-
C2D63_RS25670	152872..153387	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA2 / iroB / iroC / iroD / iroN / rmpA	1..224152	224152
	-	inside	IScluster/Tn	-	-	142307..149922	7615

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T92671 WP_011251285.1 NZ_CP026012:c148448-148137 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T92671 NZ_CP026012:c148448-148137 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT92671 WP_011251286.1 NZ_CP026012:c148140-147721 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT92671 NZ_CP026012:c148140-147721 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1