Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 878721..879283 Replicon chromosome
Accession NZ_CP025979
Organism Escherichia marmotae strain HT073016

Toxin (Protein)


Gene name higB Uniprot ID A0A7U9DG24
Locus tag C1192_RS04765 Protein ID WP_016248700.1
Coordinates 879005..879283 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag C1192_RS04760 Protein ID WP_000781370.1
Coordinates 878721..879005 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1192_RS04745 873903..876950 + 3048 WP_158650411.1 formate dehydrogenase-N subunit alpha -
C1192_RS04750 876963..877847 + 885 WP_001240589.1 formate dehydrogenase N subunit beta -
C1192_RS04755 877840..878493 + 654 WP_000045637.1 formate dehydrogenase-N subunit gamma -
C1192_RS04760 878721..879005 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
C1192_RS04765 879005..879283 - 279 WP_016248700.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C1192_RS04775 879469..880479 - 1011 WP_000642389.1 alcohol dehydrogenase AdhP -
C1192_RS04780 880613..882310 - 1698 WP_001516384.1 malate dehydrogenase -
C1192_RS04785 882466..882603 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
C1192_RS04790 882694..882909 - 216 WP_000495761.1 biofilm-dependent modulation protein -
C1192_RS04795 883232..883663 + 432 WP_038355863.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10623.19 Da        Isoelectric Point: 6.8682

>T92610 WP_016248700.1 NZ_CP025979:c879283-879005 [Escherichia marmotae]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDEIWTISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T92610 NZ_CP025979:c879283-879005 [Escherichia marmotae]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTAATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGAAATTTGGACGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT92610 WP_000781370.1 NZ_CP025979:c879005-878721 [Escherichia marmotae]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT92610 NZ_CP025979:c879005-878721 [Escherichia marmotae]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACACCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9DG24

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References