Detailed information of TA system TA092577

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79068..79338
• Replicon: plasmid pKPGD4
• Accession: NZ_CP025952
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain GD4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CXZ11_RS28625
• Protein ID: WP_001312861.1
• Coordinates: 79180..79338 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	79068..79131 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXZ11_RS27740	74779..75306	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
CXZ11_RS27745	75364..75597	+	234	WP_000006003.1	DUF905 family protein	-
CXZ11_RS27750	75658..77681	+	2024	Protein_96	ParB/RepB/Spo0J family partition protein	-
CXZ11_RS27755	77750..78184	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CXZ11_RS27760	78181..78900	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	78912..79136	+	225	NuclAT_0	-	-
-	78912..79136	+	225	NuclAT_0	-	-
-	78912..79136	+	225	NuclAT_0	-	-
-	78912..79136	+	225	NuclAT_0	-	-
-	79068..79131	-	64	-	-	Antitoxin
CXZ11_RS28625	79180..79338	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CXZ11_RS29245	79576..79953	-	378	Protein_100	hypothetical protein	-
CXZ11_RS27785	80253..80549	+	297	WP_001272251.1	hypothetical protein	-
CXZ11_RS27790	80660..81481	+	822	WP_001234445.1	DUF945 domain-containing protein	-
CXZ11_RS27795	81778..82425	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
CXZ11_RS27800	82702..83085	+	384	WP_000124981.1	relaxosome protein TraM	-
CXZ11_RS27805	83276..83962	+	687	WP_015059009.1	PAS domain-containing protein	-
CXZ11_RS27810	84056..84283	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA2 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2	-	1..170821	170821

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T92577 WP_001312861.1 NZ_CP025952:79180-79338 [Klebsiella pneumoniae subsp. pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T92577 NZ_CP025952:79180-79338 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT92577 NZ_CP025952:c79131-79068 [Klebsiella pneumoniae subsp. pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin