Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4282612..4283258 Replicon chromosome
Accession NZ_CP025951
Organism Klebsiella pneumoniae subsp. pneumoniae strain GD4

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag CXZ11_RS21385 Protein ID WP_002920560.1
Coordinates 4282612..4282959 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag CXZ11_RS21390 Protein ID WP_002920557.1
Coordinates 4282959..4283258 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXZ11_RS21375 4278538..4279971 + 1434 WP_002920564.1 glycogen synthase GlgA -
CXZ11_RS21380 4279989..4282436 + 2448 WP_002920561.1 glycogen phosphorylase -
CXZ11_RS21385 4282612..4282959 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CXZ11_RS21390 4282959..4283258 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
CXZ11_RS21395 4283321..4284829 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
CXZ11_RS21400 4285034..4285363 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
CXZ11_RS21405 4285414..4286244 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
CXZ11_RS21410 4286294..4287052 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T92574 WP_002920560.1 NZ_CP025951:4282612-4282959 [Klebsiella pneumoniae subsp. pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T92574 NZ_CP025951:4282612-4282959 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT92574 WP_002920557.1 NZ_CP025951:4282959-4283258 [Klebsiella pneumoniae subsp. pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT92574 NZ_CP025951:4282959-4283258 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References