Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1914524..1915026 Replicon chromosome
Accession NZ_CP025950
Organism Escherichia coli strain SCEC020023

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag C1A20_RS10665 Protein ID WP_000767831.1
Coordinates 1914772..1915026 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C1A20_RS10660 Protein ID WP_001259255.1
Coordinates 1914524..1914775 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1A20_RS10635 1909701..1910768 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C1A20_RS10640 1910768..1911838 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
C1A20_RS10645 1911835..1913139 - 1305 WP_001446927.1 histidinol dehydrogenase -
C1A20_RS10650 1913145..1914044 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C1A20_RS10655 1914190..1914240 - 51 WP_001364200.1 his operon leader peptide -
C1A20_RS10660 1914524..1914775 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C1A20_RS10665 1914772..1915026 + 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C1A20_RS10670 1915109..1915933 + 825 WP_000754737.1 SDR family oxidoreductase -
C1A20_RS10675 1915979..1916908 + 930 WP_024195433.1 LysR family transcriptional regulator -
C1A20_RS10680 1917123..1917185 + 63 WP_010723108.1 membrane protein YoeI -
C1A20_RS10685 1917175..1918533 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C1A20_RS10690 1918712..1919770 + 1059 WP_000492321.1 transport protein YeeE -
C1A20_RS10695 1919784..1920011 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - galF / cpsACP / wzi / wza / gnd / wbtL / ugd / fyuA 1739735..1975168 235433


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T92540 WP_000767831.1 NZ_CP025950:1914772-1915026 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T92540 NZ_CP025950:1914772-1915026 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92540 WP_001259255.1 NZ_CP025950:1914524-1914775 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92540 NZ_CP025950:1914524-1914775 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References