Detailed information of TA system TA092534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 606978..607777
• Replicon: chromosome
• Accession: NZ_CP025950
• Organism: Escherichia coli strain SCEC020023

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: D3GWU5
• Locus tag: C1A20_RS04440
• Protein ID: WP_000347272.1
• Coordinates: 606978..607442 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: C1A20_RS04445
• Protein ID: WP_001307405.1
• Coordinates: 607442..607777 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1A20_RS04410	601979..602413	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
C1A20_RS04415	602431..603309	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C1A20_RS04420	603299..604078	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C1A20_RS04425	604089..604562	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C1A20_RS04430	604585..605865	-	1281	WP_001521382.1	tagatose-bisphosphate aldolase subunit KbaZ	-
C1A20_RS04435	606114..606923	+	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
C1A20_RS04440	606978..607442	-	465	WP_000347272.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C1A20_RS04445	607442..607777	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C1A20_RS04450	607926..608819	-	894	Protein_574	UxaA family hydrolase	-
C1A20_RS04455	608854..609822	+	969	WP_000654804.1	IS5-like element IS903B family transposase	-
C1A20_RS04460	609880..610563	-	684	Protein_576	UxaA family hydrolase	-
C1A20_RS04465	610938..612272	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	608899..609822	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17832.26 Da
• Isoelectric Point: 9.6924

>T92534 WP_000347272.1 NZ_CP025950:c607442-606978 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Nucleotide

• Length: 465 bp

>T92534 NZ_CP025950:c607442-606978 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT92534 WP_001307405.1 NZ_CP025950:c607777-607442 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT92534 NZ_CP025950:c607777-607442 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829KUD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7