Detailed information of TA system TA092532

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72217..72470
• Replicon: plasmid pCTXM55_020023
• Accession: NZ_CP025949
• Organism: Escherichia coli strain SCEC020023

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: C1A20_RS01465
• Protein ID: WP_001312851.1
• Coordinates: 72321..72470 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	72217..72276 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1A20_RS01435	68944..69690	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
C1A20_RS01440	69745..70305	+	561	WP_000139328.1	fertility inhibition protein FinO	-
C1A20_RS01445	70437..70637	+	201	WP_015059022.1	hypothetical protein	-
C1A20_RS01450	71023..71622	+	600	WP_032083981.1	hypothetical protein	-
C1A20_RS01455	71786..72016	+	231	WP_001736714.1	hypothetical protein	-
-	72217..72276	-	60	NuclAT_1	-	Antitoxin
-	72217..72276	-	60	NuclAT_1	-	Antitoxin
-	72217..72276	-	60	NuclAT_1	-	Antitoxin
-	72217..72276	-	60	NuclAT_1	-	Antitoxin
C1A20_RS01465	72321..72470	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
C1A20_RS01470	72754..73002	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-IIa / blaTEM-1B / rmtB / blaCTX-M-55	-	1..73313	73313

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T92532 WP_001312851.1 NZ_CP025949:72321-72470 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T92532 NZ_CP025949:72321-72470 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT92532 NZ_CP025949:c72276-72217 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin