Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4289602..4290104 Replicon chromosome
Accession NZ_CP025920
Organism Escherichia coli strain 103605

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXW53_RS21135 Protein ID WP_000767829.1
Coordinates 4289850..4290104 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXW53_RS21130 Protein ID WP_001259255.1
Coordinates 4289602..4289853 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXW53_RS21105 4284780..4285847 - 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CXW53_RS21110 4285847..4286917 - 1071 WP_001099220.1 histidinol-phosphate transaminase -
CXW53_RS21115 4286914..4288218 - 1305 WP_001355813.1 histidinol dehydrogenase -
CXW53_RS21120 4288224..4289123 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CXW53_RS21125 4289269..4289319 - 51 WP_001364200.1 his operon leader peptide -
CXW53_RS21130 4289602..4289853 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXW53_RS21135 4289850..4290104 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXW53_RS21140 4290187..4291011 + 825 WP_000754737.1 SDR family oxidoreductase -
CXW53_RS21145 4291057..4291986 + 930 WP_000803360.1 LysR family transcriptional regulator -
CXW53_RS21150 4292201..4292263 + 63 WP_010723108.1 membrane protein YoeI -
CXW53_RS21155 4292253..4293611 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXW53_RS21160 4293790..4294848 + 1059 WP_000492305.1 transport protein YeeE -
CXW53_RS21165 4294862..4295089 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92482 WP_000767829.1 NZ_CP025920:4289850-4290104 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92482 NZ_CP025920:4289850-4290104 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92482 WP_001259255.1 NZ_CP025920:4289602-4289853 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92482 NZ_CP025920:4289602-4289853 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References