Detailed information of TA system TA092477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2989061..2989860
• Replicon: chromosome
• Accession: NZ_CP025920
• Organism: Escherichia coli strain 103605

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: CXW53_RS14810
• Protein ID: WP_000347267.1
• Coordinates: 2989061..2989525 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: CXW53_RS14815
• Protein ID: WP_001307405.1
• Coordinates: 2989525..2989860 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW53_RS14780	2984062..2984496	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
CXW53_RS14785	2984514..2985392	-	879	WP_001355782.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CXW53_RS14790	2985382..2986161	-	780	WP_000406212.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CXW53_RS14795	2986172..2986645	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CXW53_RS14800	2986668..2987948	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CXW53_RS14805	2988197..2989006	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CXW53_RS14810	2989061..2989525	-	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CXW53_RS14815	2989525..2989860	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CXW53_RS14820	2990009..2991580	-	1572	WP_001273741.1	galactarate dehydratase	-
CXW53_RS14825	2991955..2993289	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
CXW53_RS14830	2993305..2994075	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T92477 WP_000347267.1 NZ_CP025920:c2989525-2989061 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T92477 NZ_CP025920:c2989525-2989061 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT92477 WP_001307405.1 NZ_CP025920:c2989860-2989525 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT92477 NZ_CP025920:c2989860-2989525 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7