Detailed information of TA system TA092475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2156064..2156666
• Replicon: chromosome
• Accession: NZ_CP025920
• Organism: Escherichia coli strain 103605

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CXW53_RS10730
• Protein ID: WP_000897305.1
• Coordinates: 2156355..2156666 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXW53_RS10725
• Protein ID: WP_000356397.1
• Coordinates: 2156064..2156354 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW53_RS10700	2151990..2152892	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CXW53_RS10705	2152889..2153524	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CXW53_RS10710	2153521..2154450	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CXW53_RS10715	2154780..2155022	-	243	WP_001086388.1	hypothetical protein	-
CXW53_RS10720	2155241..2155459	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CXW53_RS10725	2156064..2156354	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CXW53_RS10730	2156355..2156666	-	312	WP_000897305.1	hypothetical protein	Toxin
CXW53_RS10735	2156895..2157803	+	909	WP_001162704.1	alpha/beta hydrolase	-
CXW53_RS10740	2157867..2158808	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CXW53_RS10745	2158853..2159290	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CXW53_RS10750	2159287..2160159	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CXW53_RS10755	2160153..2160752	-	600	WP_001295269.1	glucose-1-phosphatase	-
CXW53_RS10760	2160851..2161636	-	786	WP_001662158.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T92475 WP_000897305.1 NZ_CP025920:c2156666-2156355 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T92475 NZ_CP025920:c2156666-2156355 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT92475 WP_000356397.1 NZ_CP025920:c2156354-2156064 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT92475 NZ_CP025920:c2156354-2156064 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7