Detailed information of TA system TA092464

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 208772..208997
• Replicon: chromosome
• Accession: NZ_CP025920
• Organism: Escherichia coli strain 103605

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CXW53_RS01235
• Protein ID: WP_000813254.1
• Coordinates: 208772..208927 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	208939..208997 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW53_RS01195	204583..204780	-	198	WP_000917767.1	hypothetical protein	-
CXW53_RS01200	205093..205635	-	543	WP_001398904.1	DUF1133 family protein	-
CXW53_RS01205	205644..206009	-	366	WP_001297842.1	RusA family crossover junction endodeoxyribonuclease	-
CXW53_RS01210	206010..207065	-	1056	WP_001502278.1	DUF968 domain-containing protein	-
CXW53_RS01215	207067..207345	-	279	WP_023607105.1	hypothetical protein	-
CXW53_RS01220	207412..207672	-	261	WP_000701918.1	hypothetical protein	-
CXW53_RS01225	207873..208358	-	486	WP_000818165.1	type II toxin-antitoxin system death-on-curing family toxin	-
CXW53_RS01230	208377..208556	-	180	WP_001277775.1	hypothetical protein	-
CXW53_RS01235	208772..208927	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	208939..208997	+	59	-	-	Antitoxin
CXW53_RS01240	209219..209641	-	423	WP_000160146.1	hypothetical protein	-
CXW53_RS01245	209745..210157	-	413	Protein_235	DUF4406 domain-containing protein	-
CXW53_RS01250	210160..210441	-	282	WP_001509842.1	hypothetical protein	-
CXW53_RS01255	210474..211235	-	762	WP_126130059.1	DUF1627 domain-containing protein	-
CXW53_RS01260	211257..212003	-	747	WP_000788772.1	ATP-binding protein	-
CXW53_RS01265	212009..212974	-	966	WP_000054517.1	hypothetical protein	-
CXW53_RS01270	212955..213476	-	522	WP_000705382.1	hypothetical protein	-
CXW53_RS01275	213460..213687	-	228	WP_000476985.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	171127..227629	56502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T92464 WP_000813254.1 NZ_CP025920:c208927-208772 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T92464 NZ_CP025920:c208927-208772 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT92464 NZ_CP025920:208939-208997 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin