Detailed information of TA system TA092462

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47588..47813
• Replicon: chromosome
• Accession: NZ_CP025920
• Organism: Escherichia coli strain 103605

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CXW53_RS00340
• Protein ID: WP_000813254.1
• Coordinates: 47588..47743 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	47755..47813 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW53_RS27045	42634..42795	-	162	WP_001355909.1	hypothetical protein	-
CXW53_RS00290	42792..42980	-	189	WP_000874243.1	DUF1737 domain-containing protein	-
CXW53_RS00295	43241..43576	+	336	WP_000871291.1	anti-adapter protein IraM	-
CXW53_RS00300	43857..43988	-	132	WP_000562553.1	DUF3927 family protein	-
CXW53_RS00320	44884..45705	-	822	WP_000762892.1	antitermination protein	-
CXW53_RS00325	45702..46082	-	381	WP_000140028.1	RusA family crossover junction endodeoxyribonuclease	-
CXW53_RS00330	46083..47141	-	1059	WP_001736257.1	DUF968 domain-containing protein	-
CXW53_RS00335	47143..47421	-	279	WP_001410105.1	hypothetical protein	-
CXW53_RS00340	47588..47743	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	47755..47813	+	59	-	-	Antitoxin
CXW53_RS00350	48368..49177	+	810	WP_000161640.1	hypothetical protein	-
CXW53_RS00355	49324..49746	-	423	WP_001151221.1	DUF977 family protein	-
CXW53_RS00360	49787..50857	-	1071	WP_089516821.1	phage replisome organizer	-
CXW53_RS00365	50929..51354	-	426	WP_000693867.1	Rha family transcriptional regulator	-
CXW53_RS00370	51338..51581	-	244	Protein_64	hypothetical protein	-
CXW53_RS00375	51973..52311	+	339	WP_001397087.1	peptidase S24-like family protein	-
CXW53_RS00380	52604..52759	+	156	WP_071608135.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	938..62202	61264

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T92462 WP_000813254.1 NZ_CP025920:c47743-47588 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T92462 NZ_CP025920:c47743-47588 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT92462 NZ_CP025920:47755-47813 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin