Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4255192..4255694 Replicon chromosome
Accession NZ_CP025916
Organism Escherichia coli strain 120899

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXW56_RS20935 Protein ID WP_000767829.1
Coordinates 4255440..4255694 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXW56_RS20930 Protein ID WP_001259255.1
Coordinates 4255192..4255443 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXW56_RS20905 4250370..4251437 - 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CXW56_RS20910 4251437..4252507 - 1071 WP_001099220.1 histidinol-phosphate transaminase -
CXW56_RS20915 4252504..4253808 - 1305 WP_001355813.1 histidinol dehydrogenase -
CXW56_RS20920 4253814..4254713 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CXW56_RS20925 4254859..4254909 - 51 WP_001364200.1 his operon leader peptide -
CXW56_RS20930 4255192..4255443 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXW56_RS20935 4255440..4255694 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXW56_RS20940 4255777..4256601 + 825 WP_000754737.1 SDR family oxidoreductase -
CXW56_RS20945 4256647..4257576 + 930 WP_000803360.1 LysR family transcriptional regulator -
CXW56_RS20950 4257791..4257853 + 63 WP_010723108.1 membrane protein YoeI -
CXW56_RS20955 4257843..4259201 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXW56_RS20960 4259380..4260438 + 1059 WP_000492305.1 transport protein YeeE -
CXW56_RS20965 4260452..4260679 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92442 WP_000767829.1 NZ_CP025916:4255440-4255694 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92442 NZ_CP025916:4255440-4255694 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92442 WP_001259255.1 NZ_CP025916:4255192-4255443 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92442 NZ_CP025916:4255192-4255443 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References