Detailed information of TA system TA092435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2115186..2115788
• Replicon: chromosome
• Accession: NZ_CP025916
• Organism: Escherichia coli strain 120899

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CXW56_RS10485
• Protein ID: WP_000897305.1
• Coordinates: 2115477..2115788 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXW56_RS10480
• Protein ID: WP_000356397.1
• Coordinates: 2115186..2115476 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW56_RS10455	2111112..2112014	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CXW56_RS10460	2112011..2112646	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CXW56_RS10465	2112643..2113572	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CXW56_RS10470	2113902..2114144	-	243	WP_001086388.1	hypothetical protein	-
CXW56_RS10475	2114363..2114581	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CXW56_RS10480	2115186..2115476	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CXW56_RS10485	2115477..2115788	-	312	WP_000897305.1	hypothetical protein	Toxin
CXW56_RS10490	2116017..2116925	+	909	Protein_1998	alpha/beta hydrolase	-
CXW56_RS10495	2116989..2117930	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CXW56_RS10500	2117975..2118412	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CXW56_RS10505	2118409..2119281	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CXW56_RS10510	2119275..2119874	-	600	WP_001295269.1	glucose-1-phosphatase	-
CXW56_RS10515	2119973..2120758	-	786	WP_001662158.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T92435 WP_000897305.1 NZ_CP025916:c2115788-2115477 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T92435 NZ_CP025916:c2115788-2115477 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT92435 WP_000356397.1 NZ_CP025916:c2115476-2115186 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT92435 NZ_CP025916:c2115476-2115186 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7