Detailed information of TA system TA092424

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 168094..168319
• Replicon: chromosome
• Accession: NZ_CP025916
• Organism: Escherichia coli strain 120899

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CXW56_RS00995
• Protein ID: WP_000813254.1
• Coordinates: 168094..168249 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	168261..168319 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW56_RS00955	163905..164102	-	198	WP_000917767.1	hypothetical protein	-
CXW56_RS00960	164415..164957	-	543	WP_001398904.1	DUF1133 family protein	-
CXW56_RS00965	164966..165331	-	366	WP_001297842.1	RusA family crossover junction endodeoxyribonuclease	-
CXW56_RS00970	165332..166387	-	1056	WP_001502278.1	DUF968 domain-containing protein	-
CXW56_RS00975	166389..166667	-	279	WP_023607105.1	hypothetical protein	-
CXW56_RS00980	166734..166994	-	261	WP_000701918.1	hypothetical protein	-
CXW56_RS00985	167195..167680	-	486	WP_000818165.1	type II toxin-antitoxin system death-on-curing family toxin	-
CXW56_RS00990	167699..167878	-	180	WP_001277775.1	hypothetical protein	-
CXW56_RS00995	168094..168249	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	168261..168319	+	59	-	-	Antitoxin
CXW56_RS01000	168541..168963	-	423	WP_000160146.1	hypothetical protein	-
CXW56_RS01005	169067..169479	-	413	Protein_187	DUF4406 domain-containing protein	-
CXW56_RS01010	169482..169763	-	282	WP_001509842.1	hypothetical protein	-
CXW56_RS01015	169796..170557	-	762	WP_126130059.1	DUF1627 domain-containing protein	-
CXW56_RS01020	170579..171325	-	747	WP_000788772.1	ATP-binding protein	-
CXW56_RS01025	171331..172296	-	966	WP_000054517.1	hypothetical protein	-
CXW56_RS01030	172277..172798	-	522	WP_000705382.1	hypothetical protein	-
CXW56_RS01035	172782..173009	-	228	WP_000476985.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	130449..186951	56502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T92424 WP_000813254.1 NZ_CP025916:c168249-168094 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T92424 NZ_CP025916:c168249-168094 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT92424 NZ_CP025916:168261-168319 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin