Detailed information of TA system TA092422

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 8803..9028
• Replicon: chromosome
• Accession: NZ_CP025916
• Organism: Escherichia coli strain 120899

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CXW56_RS00110
• Protein ID: WP_000813254.1
• Coordinates: 8803..8958 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	8970..9028 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW56_RS27375	3850..4011	-	162	WP_001355909.1	hypothetical protein	-
CXW56_RS00060	4008..4196	-	189	WP_000874243.1	DUF1737 domain-containing protein	-
CXW56_RS00065	4457..4791	+	335	Protein_8	anti-adapter protein IraM	-
CXW56_RS00070	5072..5203	-	132	WP_000562553.1	DUF3927 family protein	-
CXW56_RS00090	6099..6920	-	822	WP_000762892.1	antitermination protein	-
CXW56_RS00095	6917..7297	-	381	WP_000140028.1	RusA family crossover junction endodeoxyribonuclease	-
CXW56_RS00100	7298..8356	-	1059	WP_001736257.1	DUF968 domain-containing protein	-
CXW56_RS00105	8358..8636	-	279	WP_001410105.1	hypothetical protein	-
CXW56_RS00110	8803..8958	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	8970..9028	+	59	-	-	Antitoxin
CXW56_RS00120	9583..10392	+	810	WP_000161640.1	hypothetical protein	-
CXW56_RS00125	10539..10961	-	423	WP_001151221.1	DUF977 family protein	-
CXW56_RS00130	11002..12072	-	1071	WP_089516821.1	phage replisome organizer	-
CXW56_RS00135	12144..12569	-	426	WP_000693867.1	Rha family transcriptional regulator	-
CXW56_RS00140	12553..12795	-	243	WP_000747951.1	hypothetical protein	-
CXW56_RS00145	13187..13525	+	339	WP_001397087.1	peptidase S24-like family protein	-
CXW56_RS00150	13818..13973	+	156	WP_071608135.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	39..23416	23377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T92422 WP_000813254.1 NZ_CP025916:c8958-8803 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T92422 NZ_CP025916:c8958-8803 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT92422 NZ_CP025916:8970-9028 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin