Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4159974..4160476 Replicon chromosome
Accession NZ_CP025913
Organism Escherichia coli strain 203740

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXW72_RS20695 Protein ID WP_000767829.1
Coordinates 4159974..4160228 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXW72_RS20700 Protein ID WP_001259255.1
Coordinates 4160225..4160476 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXW72_RS20665 4154989..4155216 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CXW72_RS20670 4155230..4156288 - 1059 WP_000492340.1 transport protein YeeE -
CXW72_RS20675 4156467..4157825 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXW72_RS20680 4157815..4157877 - 63 WP_010723108.1 membrane protein YoeI -
CXW72_RS20685 4158092..4159021 - 930 WP_000803337.1 LysR family transcriptional regulator -
CXW72_RS20690 4159067..4159891 - 825 WP_000754737.1 SDR family oxidoreductase -
CXW72_RS20695 4159974..4160228 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXW72_RS20700 4160225..4160476 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXW72_RS20705 4160759..4160809 + 51 WP_001364200.1 his operon leader peptide -
CXW72_RS20710 4160955..4161854 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CXW72_RS20715 4161860..4163158 + 1299 WP_001735396.1 histidinol dehydrogenase -
CXW72_RS20720 4163161..4164231 + 1071 WP_000108954.1 histidinol-phosphate transaminase -
CXW72_RS20725 4164231..4165298 + 1068 WP_000080145.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92421 WP_000767829.1 NZ_CP025913:c4160228-4159974 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92421 NZ_CP025913:c4160228-4159974 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92421 WP_001259255.1 NZ_CP025913:c4160476-4160225 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92421 NZ_CP025913:c4160476-4160225 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References