Detailed information of TA system TA092409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2177337..2178031
• Replicon: chromosome
• Accession: NZ_CP025913
• Organism: Escherichia coli strain 203740

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CXW72_RS10535
• Protein ID: WP_001263493.1
• Coordinates: 2177633..2178031 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CXW72_RS10530
• Protein ID: WP_000554757.1
• Coordinates: 2177337..2177630 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW72_RS10510	2172969..2173466	+	498	WP_000006242.1	REP-associated tyrosine transposase RayT	-
CXW72_RS10515	2173690..2175402	-	1713	Protein_2006	flagellar biosynthesis protein FlhA	-
CXW72_RS10520	2175374..2176159	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
CXW72_RS10525	2176230..2177285	+	1056	WP_001226164.1	DNA polymerase IV	-
CXW72_RS10530	2177337..2177630	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CXW72_RS10535	2177633..2178031	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CXW72_RS10540	2178041..2178493	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CXW72_RS10545	2178739..2178942	+	204	Protein_2012	RNA ligase RtcB family protein	-
CXW72_RS10550	2178941..2179462	+	522	Protein_2013	peptide chain release factor H	-
CXW72_RS10555	2179519..2180976	-	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
CXW72_RS10560	2181237..2181695	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CXW72_RS10565	2181787..2183031	+	1245	WP_000189541.1	esterase FrsA	-
-	2182291..2182371	+	81	NuclAT_11	-	-
-	2182291..2182371	+	81	NuclAT_11	-	-
-	2182291..2182371	+	81	NuclAT_11	-	-
-	2182291..2182371	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T92409 WP_001263493.1 NZ_CP025913:2177633-2178031 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T92409 NZ_CP025913:2177633-2178031 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT92409 WP_000554757.1 NZ_CP025913:2177337-2177630 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT92409 NZ_CP025913:2177337-2177630 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6