Detailed information of TA system TA092395

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4123340..4123565
• Replicon: chromosome
• Accession: NZ_CP025910
• Organism: Escherichia coli strain 204446

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CXW77_RS20460
• Protein ID: WP_000813254.1
• Coordinates: 4123340..4123495 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	4123507..4123565 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW77_RS20420	4119151..4119348	-	198	WP_000917767.1	hypothetical protein	-
CXW77_RS20425	4119661..4120203	-	543	WP_001398904.1	DUF1133 family protein	-
CXW77_RS20430	4120212..4120577	-	366	WP_001297842.1	RusA family crossover junction endodeoxyribonuclease	-
CXW77_RS20435	4120578..4121633	-	1056	WP_001502278.1	DUF968 domain-containing protein	-
CXW77_RS20440	4121635..4121913	-	279	WP_023607105.1	hypothetical protein	-
CXW77_RS20445	4121980..4122240	-	261	WP_000701918.1	hypothetical protein	-
CXW77_RS20450	4122441..4122926	-	486	WP_000818165.1	type II toxin-antitoxin system death-on-curing family toxin	-
CXW77_RS20455	4122945..4123124	-	180	WP_001277775.1	hypothetical protein	-
CXW77_RS20460	4123340..4123495	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	4123507..4123565	+	59	-	-	Antitoxin
CXW77_RS20465	4123787..4124209	-	423	WP_000160146.1	hypothetical protein	-
CXW77_RS20470	4124313..4124725	-	413	Protein_3928	DUF4406 domain-containing protein	-
CXW77_RS20475	4124728..4125009	-	282	WP_001509842.1	hypothetical protein	-
CXW77_RS20480	4125042..4125803	-	762	WP_126130059.1	DUF1627 domain-containing protein	-
CXW77_RS20485	4125825..4126571	-	747	WP_000788772.1	ATP-binding protein	-
CXW77_RS20490	4126577..4127542	-	966	WP_000054517.1	hypothetical protein	-
CXW77_RS20495	4127523..4128044	-	522	WP_000705382.1	hypothetical protein	-
CXW77_RS20500	4128028..4128255	-	228	WP_000476985.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4082949..4139296	56347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T92395 WP_000813254.1 NZ_CP025910:c4123495-4123340 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T92395 NZ_CP025910:c4123495-4123340 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT92395 NZ_CP025910:4123507-4123565 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin