Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3114787..3115289 Replicon chromosome
Accession NZ_CP025910
Organism Escherichia coli strain 204446

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXW77_RS15190 Protein ID WP_000767829.1
Coordinates 3115035..3115289 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXW77_RS15185 Protein ID WP_001259255.1
Coordinates 3114787..3115038 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXW77_RS15160 3109965..3111032 - 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CXW77_RS15165 3111032..3112102 - 1071 WP_001099220.1 histidinol-phosphate transaminase -
CXW77_RS15170 3112099..3113403 - 1305 WP_001355813.1 histidinol dehydrogenase -
CXW77_RS15175 3113409..3114308 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CXW77_RS15180 3114454..3114504 - 51 WP_001364200.1 his operon leader peptide -
CXW77_RS15185 3114787..3115038 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXW77_RS15190 3115035..3115289 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXW77_RS15195 3115372..3116196 + 825 WP_000754737.1 SDR family oxidoreductase -
CXW77_RS15200 3116242..3117171 + 930 WP_000803360.1 LysR family transcriptional regulator -
CXW77_RS15205 3117386..3117448 + 63 WP_010723108.1 membrane protein YoeI -
CXW77_RS15210 3117438..3118796 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXW77_RS15215 3118975..3120033 + 1059 WP_000492305.1 transport protein YeeE -
CXW77_RS15220 3120047..3120274 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92380 WP_000767829.1 NZ_CP025910:3115035-3115289 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92380 NZ_CP025910:3115035-3115289 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92380 WP_001259255.1 NZ_CP025910:3114787-3115038 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92380 NZ_CP025910:3114787-3115038 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References