Detailed information of TA system TA092373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 981249..981851
• Replicon: chromosome
• Accession: NZ_CP025910
• Organism: Escherichia coli strain 204446

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CXW77_RS04785
• Protein ID: WP_000897305.1
• Coordinates: 981540..981851 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXW77_RS04780
• Protein ID: WP_000356397.1
• Coordinates: 981249..981539 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXW77_RS04755	977175..978077	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CXW77_RS04760	978074..978709	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CXW77_RS04765	978706..979635	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CXW77_RS04770	979965..980207	-	243	WP_001086388.1	hypothetical protein	-
CXW77_RS04775	980426..980644	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CXW77_RS04780	981249..981539	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CXW77_RS04785	981540..981851	-	312	WP_000897305.1	hypothetical protein	Toxin
CXW77_RS04790	982080..982988	+	909	WP_001162704.1	alpha/beta hydrolase	-
CXW77_RS04795	983052..983993	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CXW77_RS04800	984038..984475	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CXW77_RS04805	984472..985344	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CXW77_RS04810	985338..985937	-	600	WP_001295269.1	glucose-1-phosphatase	-
CXW77_RS04815	986036..986821	-	786	WP_001662158.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T92373 WP_000897305.1 NZ_CP025910:c981851-981540 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T92373 NZ_CP025910:c981851-981540 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT92373 WP_000356397.1 NZ_CP025910:c981539-981249 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT92373 NZ_CP025910:c981539-981249 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7