Detailed information of TA system TA092358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2416106..2416608
• Replicon: chromosome
• Accession: NZ_CP025903
• Organism: Escherichia coli strain 300709

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: CXV43_RS12285
• Protein ID: WP_000767835.1
• Coordinates: 2416106..2416360 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: CXV43_RS12290
• Protein ID: WP_001259255.1
• Coordinates: 2416357..2416608 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV43_RS12260	2411660..2412357	-	698	Protein_2381	IS1 family transposase	-
CXV43_RS12265	2412599..2413957	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
CXV43_RS12270	2413947..2414009	-	63	WP_010723108.1	membrane protein YoeI	-
CXV43_RS12275	2414224..2415153	-	930	WP_000803351.1	LysR family transcriptional regulator	-
CXV43_RS12280	2415199..2416023	-	825	WP_000754737.1	SDR family oxidoreductase	-
CXV43_RS12285	2416106..2416360	-	255	WP_000767835.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
CXV43_RS12290	2416357..2416608	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
CXV43_RS12295	2416892..2416942	+	51	WP_001364200.1	his operon leader peptide	-
CXV43_RS12300	2417088..2417987	+	900	WP_000131774.1	ATP phosphoribosyltransferase	-
CXV43_RS12305	2417993..2419297	+	1305	WP_001737303.1	histidinol dehydrogenase	-
CXV43_RS12310	2419294..2420364	+	1071	WP_000108941.1	histidinol-phosphate transaminase	-
CXV43_RS12315	2420364..2421431	+	1068	WP_000080072.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10169.51 Da
• Isoelectric Point: 7.2751

>T92358 WP_000767835.1 NZ_CP025903:c2416360-2416106 [Escherichia coli]
VKLIWSEESWDDYLYWQETDNRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T92358 NZ_CP025903:c2416360-2416106 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAATCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT92358 WP_001259255.1 NZ_CP025903:c2416608-2416357 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT92358 NZ_CP025903:c2416608-2416357 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LNR0