Detailed information of TA system TA092344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 476080..476774
• Replicon: chromosome
• Accession: NZ_CP025903
• Organism: Escherichia coli strain 300709

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: CXV43_RS02325
• Protein ID: WP_001263489.1
• Coordinates: 476376..476774 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: CXV43_RS02320
• Protein ID: WP_001412607.1
• Coordinates: 476080..476373 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV43_RS02295	471897..472364	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
CXV43_RS02300	472384..473100	+	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
CXV43_RS02305	473113..473976	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
CXV43_RS02310	473979..474902	+	924	WP_001232546.1	putative lateral flagellar export/assembly protein LafU	-
CXV43_RS02315	474973..476028	+	1056	WP_001226164.1	DNA polymerase IV	-
CXV43_RS02320	476080..476373	+	294	WP_001412607.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CXV43_RS02325	476376..476774	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CXV43_RS02330	476784..477236	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
CXV43_RS02335	477554..477760	+	207	Protein_453	RtcB family protein	-
CXV43_RS02340	477756..478277	+	522	Protein_454	peptide chain release factor H	-
CXV43_RS02345	478334..479791	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
CXV43_RS02350	480052..480510	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	481106..481186	+	81	NuclAT_12	-	-
-	481106..481186	+	81	NuclAT_12	-	-
-	481106..481186	+	81	NuclAT_12	-	-
-	481106..481186	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T92344 WP_001263489.1 NZ_CP025903:476376-476774 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T92344 NZ_CP025903:476376-476774 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11199.75 Da
• Isoelectric Point: 5.2170

>AT92344 WP_001412607.1 NZ_CP025903:476080-476373 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDLKE

Nucleotide

• Length: 294 bp

>AT92344 NZ_CP025903:476080-476373 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACCTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1